Sulphide Donor Exhibits Cytoprotective and Antioxidative Activity in UV-induced HaCaT Cell Lines

Oxidative stress due to excessive of reactive oxygen species (ROS) in the body is commonly associated as one underlying mechanisms ultraviolet radiation (UV)-induced damage skin. Reactive sulphur (RSS) has been implicated with potent antioxidant properties and involves modulating cellular signalling transduction pathway. In this current study, potential involvement RSS alleviating UV-induced HaCaT keratinocytes was explored. We first established suitable dose UVA UVB that can depressed cells population, 80 mJ/cm2 10 mJ/cm2, respectively. The sulphide donor, NaHS, also tested for its cytotoxicity profile, 200 µM considered non-toxic concentration cells. Our data showed pre-treating NaHS decreased detrimental effects UV especially on demonstrated by MTT assay. work, we used 2’,7’-dichlorofluorescin-diacetate (DCFH-DA), a useful indicator ROS, regard determining NaHS. demonstrate significantly suppressed intracellular ROS production induced both irradiation comparison absence (p<0.05). conclusion, supplementation biological condition particularly provides protective effect skin against diminishing formation ROS. Nonetheless, study preliminary insight role damages vitro model which be further expand precision technique future.